All procedures are discussed in detail in sections indicated on the flow chart. Shipments of MAB’s are accompanied by a detailed certificate of analysis.A customized, multistep purification process is performed to meet MAB specifications.Custom MAB production is offered in ascites and, as an alternate method to in vivo production, SLI can propagate a large number of antibody producing cells in vitro using bioreactors.Cell line drift, one of the greatest dangers encountered by a cell line, can be prevented with close monitoring and cell line certification.SLI can offer cell line optimization for maximum MAB production.Careful shipment ensures the safety of the cell line. Alternatively, an existing hybridoma is shipped to SLI utilizing SLI shipping procedures.SLI (Scantibodies Laboratory, Inc.) offers hybridoma development service if there is no existing hybridoma available to the customer. The first step in MAB production is hybridoma development.Limits in hybridoma antibody production and losses incurred during the purification process can account for dramatically increased cost. When a price quote is based on forms other than purified MAB (i.e., number of mice or bioreactor, volume of ascites, etc.), the final MAB price is indeterminate. No Risk Plan (per milligram of antibody in purified form)īy purchasing the MAB on the basis of purified, functional antibody, risk to the customer is eliminated. Per milligram of antibody in ascites or supernatant media (non-purified form)ģ. The price can be based on any one of the following:Ģ. There are three types of price plans available for custom MAB (Monoclonal Antibody) production. ![]() Custom in vitro monoclonal antibody production is performed in different types of bioreactors. Other strains of mice (Swiss Webster, CAF-1, ICR, etc.) are also available for antibody production. At Scantibodies, BALB/c mice are maintained in a breeding colony with a population of 100,000 mice. For cost, speed and scale up reasons, the in vivo type has been the most popular method of choice.Ĭustom in vivo monoclonal antibody production is accomplished using a wide variety of cell lines. There are two types of custom monoclonal antibody production, in vivo (typically in mice), and in vitro (using bioreactors). The following is a description of the overall system and procedures used when Scantibodies performs custom monoclonal antibody (MAB) production. Over the past ten years, Scantibodies has built up an efficient, cost effective system of manufacturing monoclonal antibodies on a contract basis.
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